Expressing:Article Title: Pennogenin-3- O -α- L -Rhamnopyranosyl-(1→2)-[α- L -Rhamnopyranosyl-(1→3)]-β- D -Glucopyranoside (Spiroconazol A) Isolated from Dioscorea bulbifera L. var. sativa Induces Autophagic Cell Death by p38 MAPK Activation in NSCLC Cells
Article Snippet: .. The cDNA of LC3-II was inserted into the pEGFP-VSVG mammalian expression vector (Addgene, Cambridge, MA, USA). .. After the transfection of pEGFP-VSVG and pEGFP-LC3 into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA), transfected cells were treated with spiroconazol A for 24 h. Then, cells were fixed with 4% paraformaldehyde for 5 min. After cell washing with PBS three times, cells were stained with 20 μL of PI (100 μg/mL) and observed by using a confocal fluorescence microscope (Carl Zeiss, Oberkochen, Germany).
Sequencing:Article Title: Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor
Article Snippet: .. 8 , 22 VSVG cDNA sequence was isolated by restriction digestion from pEGFP-VSVG vector (Addgene 11912, deposited by Jennifer Lippincott-Schwartz) purchased from Addgene (a plasmid repository; Cambridge, MA) and cloned into pEGFP-C3 vector (Takara Bio, Mountain View, CA). .. GALT-CFP plasmid was prepared as follows: E2-Crimson-tagged β1, 4 galactosyltransferase 1 (GalT) with KDEL ER targeting sequence deleted was created in the pECFP vector by subcloning the E2-Crimson cassette in place of enhanced cyan fluorescent protein cassette in the GALT-CFP plasmid (Addgene 11937, a gift from Jennifer Lippincott-Schwartz) Bioinformatics RAB1B sequence (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_030981.2","term_id":"116014337","term_text":"NM_030981.2"}} NM_030981.2 ) was obtained from the National Center for Biotechnology Information gene database.
Isolation:Article Title: Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor
Article Snippet: .. 8 , 22 VSVG cDNA sequence was isolated by restriction digestion from pEGFP-VSVG vector (Addgene 11912, deposited by Jennifer Lippincott-Schwartz) purchased from Addgene (a plasmid repository; Cambridge, MA) and cloned into pEGFP-C3 vector (Takara Bio, Mountain View, CA). .. GALT-CFP plasmid was prepared as follows: E2-Crimson-tagged β1, 4 galactosyltransferase 1 (GalT) with KDEL ER targeting sequence deleted was created in the pECFP vector by subcloning the E2-Crimson cassette in place of enhanced cyan fluorescent protein cassette in the GALT-CFP plasmid (Addgene 11937, a gift from Jennifer Lippincott-Schwartz) Bioinformatics RAB1B sequence (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_030981.2","term_id":"116014337","term_text":"NM_030981.2"}} NM_030981.2 ) was obtained from the National Center for Biotechnology Information gene database.
Plasmid Preparation:Article Title: Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor
Article Snippet: .. 8 , 22 VSVG cDNA sequence was isolated by restriction digestion from pEGFP-VSVG vector (Addgene 11912, deposited by Jennifer Lippincott-Schwartz) purchased from Addgene (a plasmid repository; Cambridge, MA) and cloned into pEGFP-C3 vector (Takara Bio, Mountain View, CA). .. GALT-CFP plasmid was prepared as follows: E2-Crimson-tagged β1, 4 galactosyltransferase 1 (GalT) with KDEL ER targeting sequence deleted was created in the pECFP vector by subcloning the E2-Crimson cassette in place of enhanced cyan fluorescent protein cassette in the GALT-CFP plasmid (Addgene 11937, a gift from Jennifer Lippincott-Schwartz) Bioinformatics RAB1B sequence (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_030981.2","term_id":"116014337","term_text":"NM_030981.2"}} NM_030981.2 ) was obtained from the National Center for Biotechnology Information gene database.
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFPtagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer LippincottSchwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions (Brody et al., 2016; Patterson et al., 2008). ..
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFP-tagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer Lippincott-Schwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions ( ; ). ..
Clone Assay:Article Title: Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor
Article Snippet: .. 8 , 22 VSVG cDNA sequence was isolated by restriction digestion from pEGFP-VSVG vector (Addgene 11912, deposited by Jennifer Lippincott-Schwartz) purchased from Addgene (a plasmid repository; Cambridge, MA) and cloned into pEGFP-C3 vector (Takara Bio, Mountain View, CA). .. GALT-CFP plasmid was prepared as follows: E2-Crimson-tagged β1, 4 galactosyltransferase 1 (GalT) with KDEL ER targeting sequence deleted was created in the pECFP vector by subcloning the E2-Crimson cassette in place of enhanced cyan fluorescent protein cassette in the GALT-CFP plasmid (Addgene 11937, a gift from Jennifer Lippincott-Schwartz) Bioinformatics RAB1B sequence (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_030981.2","term_id":"116014337","term_text":"NM_030981.2"}} NM_030981.2 ) was obtained from the National Center for Biotechnology Information gene database.
Amplification:Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFPtagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer LippincottSchwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions (Brody et al., 2016; Patterson et al., 2008). ..
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFP-tagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer Lippincott-Schwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions ( ; ). ..
Polymerase Chain Reaction:Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFPtagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer LippincottSchwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions (Brody et al., 2016; Patterson et al., 2008). ..
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFP-tagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer Lippincott-Schwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions ( ; ). ..
Transfection:Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFPtagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer LippincottSchwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions (Brody et al., 2016; Patterson et al., 2008). ..
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFP-tagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer Lippincott-Schwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions ( ; ). ..
Recombinant:Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFPtagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer LippincottSchwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions (Brody et al., 2016; Patterson et al., 2008). ..
Article Title: Thrombospondin expression in myofibers stabilizes muscle membranes
Article Snippet: .. The cDNAs of human Nell2 (Harvard Plasmids, HsCD00331038) and eGFP-tagged VSV-G-ts045 (VSVG-eGFP, Addgene: Plasmid #11912 deposited by Dr. Jennifer Lippincott-Schwartz) were amplified by PCR for insertion into the pAdenoX-CMV vector (Clontech, Mountain View, CA) and transfected into HEK cells to generate recombinant adenovirus following manufacturer’s instructions ( ; ). ..
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